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Forestry Advance Access published online on February 14, 2008
Forestry, doi:10.1093/forestry/cpm047
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© Institute of Chartered Foresters, 2008. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Artificial hybridization between Robinia pseudoacacia L. and R. pseudoacacia var. monophylla Carr

Olympia Dini-Papanastasi1,* and Filippos A. Aravanopoulos2

1 National Agricultural Research Foundation, Forest Research Institute, GR-570 06 Vassilika, Thessaloniki, Greece
2 Faculty of Forestry and Natural Environment, Aristotle University of Thessaloniki, PO Box 238, GR-541 24 Thessaloniki, Greece

* Corresponding author. E-mail: olympia{at}fri.gr


  Abstract

Results regarding artificial cross- and self-pollination between Robinia pseudoacacia L. and R. pseudoacacia var. monophylla Carr. are reported. Parental clones involved in a diallel mating scheme were selected according to leaf type phenotype and included (1) single-leaf type, (2) 25-leaf type, (3) three-leaf type and (4) common Robinia. Artificial pollination proved to be a difficult and delicate procedure. A total of 33 pollinations were made that resulted in the formation of 84 pods, of which 24 developed fully and produced 65 seeds. Fifteen seeds were germinated, resulting in the production of two putative fertile hybrids. The success of hybridization was assessed by genetic markers, while parental clones and hybrid progeny were assigned multilocus genotypes. The mode of inheritance of the leaf type trait was studied in open-pollinated families where the monophylla type was the maternal parent. The study indicated that leaf type in the monophylla variety may be associated with major gene effects.

Received 1 February 2007.
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