Skip Navigation


Forestry Advance Access originally published online on January 29, 2009
Forestry 2009 82(2):175-184; doi:10.1093/forestry/cpn052
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
82/2/175    most recent
cpn052v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Álvarez, J. M.
Right arrow Articles by Ordás, R. J.
Right arrow Search for Related Content
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Institute of Chartered Foresters, 2009. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

An improved micropropagation protocol for maritime pine (Pinus pinaster Ait.) isolated cotyledons

J. M. Álvarez1, J. Majada2 and R. J. Ordás1,*

1 Área de Fisiología Vegetal, Instituto Universitario de Biotecnología, Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n, 33071-Oviedo, Spain
2 Sección Forestal, Servicio Regional de Investigación y Desarrollo Agroalimentario de Asturias, Finca Experimental ‘La Mata’, 33820-Grado, Asturias, Spain

* Corresponding author. E-mail: rordas{at}uniovi.es


  Abstract

In this work an improved micropropagation protocol for Pinus pinaster Ait. using isolated cotyledons is presented. The variables studied during bud induction were embryo preculture, type and concentration of cytokinin and influence of illumination culture conditions. Shoot development and proliferation was obtained by transfer to hormone-free medium with activated charcoal. This protocol also describes a procedure for successful continuous multiplication using axillary shoots and brachyblasts formed during shoot development. Based on 86 per cent rooting success rate, at least 70 plantlets can be expected per seed after 16 weeks.

Received 12 December 2007.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.